镰刀菌毒素对断奶仔猪子宫组织结构、Hsp70分布和表达的影响

doi:10.11843/j.issn.0366-6964.2017.06.008

Abstract

Abstract:

The present study was aimed at investigating the effects of fusarium toxins on histological structure, Hsp70 distribution and mRNA expression levels in the uterus of post-weaning piglets. A total of 20 healthy post-weaning piglets (Duroc×Landrace×Large White) weaned at 35 d with an average body weight ((8.45±0.94)kg)were randomly allocated into 2 treatments with 10 in the control group and 10 in the fusarium toxins group. Piglets of the control group were fed a based diet only, and piglets of the fusarium toxins group were fed test diet containing fusarium toxin (0.90 mg·kg^-1 ZEN, 1.43 mg·kg^-1 DON, 5.85 mg·kg^-1 FUM) for 35 d after 7 d adaptation. Results showed that the average daily feed intake and average daily gain of piglets of the fusarium group were decreased significantly (P<0.01), however, the uterine organ index (control group: 0.91±0.03, fusarium toxins group: 1.90±0.10), the number and density of uterine glandular acini and the thickness of endometrium of postweaning piglets (control group: 1 002.55±101.22 μm, fusarium toxins group: 1 343.09±104.57 μm, P<0.01) were increased significantly, the thickeness of the myometrium was more than doubled that of the control group (control group: 355.58±28.26 μm, fusarium toxins group: 779.56±40.38 μm, P<0.01), these were extremly significant difference in comparison with the control group (P<0.01). The observation result of Hsp70 immunoreactive cells revealed that Hsp70 were mainly distributed in the cytoplasm of the endometrium epithelium and columnar glandular epithelium. Compared with the control group, the Hsp70 immunoreactivity of the fusarium toxins group was enhanced obviously (P<0.01), as well as Hsp70 positive cells of the fusarium toxins group were also found in the inner circular muscle layer of the uterus, and the IOD of Hsp70 in the uterine glandular epithelial cells was 2 times higher than that of the control group (P<0.01). The relative expression of Hsp70 mRNA in the fusarium toxins group was significantly higher than that of the control group (P<0.05). In conclusion, the present study found that the fusarium toxins could cause the oxidative stress of the uterus in post-weaning piglets, and this results suggested that the endometrial epithelium and columnar glandular epithelium could resist the effects of fusarium toxins on the normal physiological function of the uterus within a certein degree by incereasing its autocrine ability of HSP70 in piglet uterus.

CLC Number:

S852.2

SUN Wen-tao, GAO Wen-bo, DAI Mei-ling, CHEN Xiang-xing, YANG Zai-bin, JIANG Shu-zhen, YANG Wei-ren, HUANG Li-bo. Effect of Dietary Fusarium Toxin on Histological Structure, Distribution and Expression of Hsp70 in the Uterus of Post-Weaning Piglets[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(6): 1035-1043.

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Effect of Dietary Fusarium Toxin on Histological Structure, Distribution and Expression of Hsp70 in the Uterus of Post-Weaning Piglets

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